Human Lipocalin 1 ELISA Kit | MyBioSource MBS069506 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Lipocalin 1 ELISA Kit

catalog :

MBS069506

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS069506

products type :

ELISA Kit

products full name :

Human Lipocalin 1 ELISA Kit

products short name :

Lipocalin 1

other names :

lipocalin-1 isoform 1; Lipocalin-1; lipocalin-1; VEG protein; tear prealbumin; Von Ebner gland protein; lipocalin 1 (tear prealbumin); protein migrating faster than albumin; lipocalin 1; Tear lipocalin; Tlc; Tear prealbumin; TP; Von Ebner gland protein

products gene name :

LCN1

other gene names :

LCN1; LCN1; TP; TLC; PMFA; VEGP; VEGP; Tlc; TP; VEG protein

uniprot entry name :

LCN1_HUMAN

reactivity :

Human

sequence length :

176

specificity :

No significant cross-reactivity or interference between Human LCN1 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated LCN1 concentrations in Human serum, plasma and other body fluids. Using Purified Human LCN1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LCN1 and LCN1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LCN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated LCN1 concentrations in Human serum, plasma and other body fluids. Using Purified Human LCN1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LCN1 and LCN1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LCN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4504963

ncbi acc num :

NP_002288.1

ncbi gb acc num :

NM_002297.3

uniprot acc num :

P31025

ncbi mol weight :

19,250 Da

ncbi summary :

This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9. [provided by RefSeq, Nov 2011]

uniprot summary :

LCN1: Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket. Belongs to the calycin superfamily. Lipocalin family. Protein type: Secreted; Secreted, signal peptide; Inhibitor. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: extracellular space; extracellular region. Molecular Function: cysteine protease inhibitor activity. Biological Process: long-chain fatty acid transport; retinal homeostasis; sensory perception of taste; response to stimulus; proteolysis; transmembrane transport

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage