Horse Uncoupling Protein 1, Mitochondrial ELISA Kit | MyBioSource MBS066225 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Horse Uncoupling Protein 1, Mitochondrial ELISA Kit

catalog :

MBS066225

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS066225

products type :

ELISA Kit

products full name :

Horse Uncoupling Protein 1, Mitochondrial ELISA Kit

products short name :

Uncoupling Protein 1, Mitochondrial

other names :

uncoupling family protein PUMP2; Mitochondrial uncoupling protein 1; uncoupling family protein PUMP2

products gene name :

UCP1

other gene names :

PUMP1; PUMP1; ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1; ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1; ATPUMP1; ATUCP1; plant uncoupling mitochondrial protein 1; PUMP1; UCP; UCP1; UNCOUPLING PROTEIN 1; UCP1; AtPUMP1

uniprot entry name :

PUMP1_ARATH

reactivity :

Horse

sequence length :

306

specificity :

No significant cross-reactivity or interference between Horse UCP1 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated UCP1 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse UCP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add UCP1 and UCP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UCP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated UCP1 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse UCP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add UCP1 and UCP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UCP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

15232420

ncbi acc num :

NP_190979.1

ncbi gb acc num :

NM_115271.4

uniprot acc num :

O81845

ncbi mol weight :

32,662 Da

ncbi pathways :

Metabolism Pathway (1007804); Mitochondrial Uncoupling Proteins Pathway (1007939); Respiratory Electron Transport, ATP Synthesis By Chemiosmotic Coupling, And Heat Production By Uncoupling Proteins. Pathway (1007937); The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway (1007931); The Fatty Acid Cycling Model Pathway (1007940); The Proton Buffering Model Pathway (1007941)

ncbi summary :

Member of Uncoupling protein PUMP2 family. Encodes a mitochondrial uncoupling protein AtUCP1 involved in maintain the redox poise of the mitochondrial electron transport chain to facilitate photosynthetic metabolism. Disruption of UCP1 results in a photosynthetic phenotype. Specifically there is a restriction in photorespiration with a decrease in the rate of oxidation of photorespiratory glycine in the mitochondrion. This change leads to an associated reduced photosynthetic carbon assimilation rate.

uniprot summary :

Function: PUMPS are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. This leads to a decrease in the efficiency of oxidative phosphorylation and an increase in heat production. Is involved in protecting plant cells against oxidative stress damage and maintaining the redox balance of the mitochondrial electron transport chain to facilitate photosynthetic metabolism. May play a regulatory role during photorespiration. Ref.8 Ref.9 Ref.12 Ref.13 Ref.15 Ref.16. Subcellular location: Mitochondrion inner membrane; Multi-pass membrane protein . By similarity. Tissue specificity: Widely expressed. Ref.1 Ref.12. Induction: By cold stress, high light and chloramphenicol. Ref.1 Ref.10 Ref.14. Disruption phenotype: Slight reduction of shoot mass. Ref.12. Miscellaneous: Over-expression of Arabidopsis UCP1 in tobacco plants increases tolerance to oxidative stress caused by exogenous hydrogen peroxide, or by drought and salt (Ref.9). Sequence similarities: Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]Contains 3 Solcar repeats. Sequence caution: The sequence BAD95028.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage