ELISA/assay

Camel Estrogens ELISA Kit

MBS062777

48-Strip-Wells

435 USD

ELISA Kit

Camel Estrogens ELISA Kit

Estrogens

Estrogen receptor; Estrogen receptor; estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; nuclear receptor subfamily 3 group A member 1; estrogen receptor 1; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1

ES

ESR1; ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1; ESR; NR3A1; ER

ESR1_HUMAN

Camel

595

No significant cross-reactivity or interference between Camel ES and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 15.6 pg/ml - 500 pg/ml. Sensitivity: 2.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ES concentrations in Camel serum, plasma and other body fluids. Using Purified Camel ES antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ES and ES antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ES in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ES concentrations in Camel serum, plasma and other body fluids. Using Purified Camel ES antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ES and ES antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ES in the samples is then determined by comparing the O.D. of the samples to the standard curve.

544257

P03372.2

P03372

66,216 Da

Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213307); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276); Estrogen Signaling Pathway (799177); Estrogen Signaling Pathway (198863); Estrogen Signaling Pathway (799197); FOXA1 Transcription Factor Network Pathway (137979); FOXM1 Transcription Factor Network Pathway (137935); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Integrated Breast Cancer Pathway (219801)

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described. Protein type: Nuclear receptor; DNA-binding. Chromosomal Location of Human Ortholog: 6q25.1. Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; integral to membrane; nucleus. Molecular Function: identical protein binding; protein binding; enzyme binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; steroid hormone receptor activity; estrogen response element binding; chromatin binding; transcription factor activity; transcription factor binding; steroid binding. Biological Process: steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; positive regulation of nitric oxide biosynthetic process; estrogen receptor signaling pathway; transcription, DNA-dependent; positive regulation of nitric-oxide synthase activity; negative regulation of transcription factor activity; antral ovarian follicle growth; male gonad development; androgen metabolic process; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; uterus development; signal transduction; response to estradiol stimulus; chromatin remodeling; regulation of apoptosis; positive regulation of fibroblast proliferation; elevation of cytosolic calcium ion concentration; regulation of transcription, DNA-dependent; response to estrogen stimulus; epithelial cell development; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of transcription factor activity; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter. Disease: Breast Cancer; Migraine With Or Without Aura, Susceptibility To, 1; Myocardial Infarction, Susceptibility To; Estrogen Resistance

48-Strip-Wells

435 USD

96-Strip-Wells

600