catalog number :
MBS062722
products type :
ELISA Kit
products full name :
Rat Semaphorin 3E ELISA Kit
products short name :
Semaphorin 3E
products gene name :
SEMA3E
specificity :
No significant cross-reactivity or interference between Rat SEMA3E and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 ng/ml - 200 ng/ml. Sensitivity: 1.0 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SEMA3E concentrations in Rat serum, plasma and other body fluids. Using Purified Rat SEMA3E antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SEMA3E and SEMA3E antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SEMA3E in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SEMA3E concentrations in Rat serum, plasma and other body fluids. Using Purified Rat SEMA3E antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SEMA3E and SEMA3E antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SEMA3E in the samples is then determined by comparing the O.D. of the samples to the standard curve.