ELISA/assay

Rabbit Prekallikrein ELISA Kit

MBS057847

48-Strip-Wells

435 USD

ELISA Kit

Rabbit Prekallikrein ELISA Kit

Prekallikrein

plasma kallikrein preproprotein; Plasma kallikrein; plasma kallikrein; kininogenin; Fletcher factor; plasma prekallikrein; plasma kallikrein heavy chain; plasma kallikrein light chain; kallikrein B, plasma (Fletcher factor) 1; Fletcher factor; Kininogenin; Plasma prekallikrein

PK

KLKB1; KLKB1; PPK; KLK3; KLK3

KLKB1_HUMAN

Rabbit

638

No significant cross-reactivity or interference between Rabbit PK and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 mumol/L - 200 mumol/L. Sensitivity: 1.0 mumol/L.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PK concentrations in Rabbit serum, plasma and other body fluids. Using Purified Rabbit PK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PK and PK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PK in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PK concentrations in Rabbit serum, plasma and other body fluids. Using Purified Rabbit PK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PK and PK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

78191798

NP_000883.2

NM_000892.3

P03952

71,370 Da

Activation Of Matrix Metalloproteinases Pathway (576264); Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028); Intrinsic Pathway (106059)

Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients. [provided by RefSeq, Jul 2008]

KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily. Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.21.34. Chromosomal Location of Human Ortholog: 4q35. Cellular Component: extracellular space; plasma membrane; extracellular region. Molecular Function: protein binding; serine-type endopeptidase activity. Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; fibrinolysis; positive regulation of fibrinolysis; zymogen activation; proteolysis; blood coagulation; blood coagulation, intrinsic pathway; plasminogen activation; Factor XII activation. Disease: Prekallikrein Deficiency

48-Strip-Wells

435 USD

96-Strip-Wells

600