ELISA/assay

Bovine Preprovasopressin ELISA Kit

MBS054157

48-Strip-Wells

435 USD

ELISA Kit

Bovine Preprovasopressin ELISA Kit

Preprovasopressin

VP, partial; Capsid protein VP1/VP2; Coat protein VP1/VP2

VP

VP

CAPSD_AADNV

Bovine

363

No significant cross-reactivity or interference between Bovine VP and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated VP concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine VP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VP and VP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) are less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated VP concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine VP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VP and VP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

1334802

CAA52901.1

Q90187

40,942 Da

Function: Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of size variants of the capsid proteins, which differ in the N-terminushe capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis . By similarity. Ref.2. Subcellular location: Virion . Potential. Sequence similarities: Belongs to the parvoviridae capsid protein family. Sequence caution: The sequence CAA52901.1 differs from that shown. Reason: Erroneous initiation.

48-Strip-Wells

435 USD

96-Strip-Wells

600