ELISA/assay

Porcine Poly ADP Ribose Polymerase (PARP) ELISA Kit

MBS051840

48-Strip-Wells

435 USD

ELISA Kit

Porcine Poly ADP Ribose Polymerase (PARP) ELISA Kit

Poly ADP Ribose Polymerase (PARP)

poly; Poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); poly (ADP-ribose) polymerase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1

PARP

PARP1; PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1

PARP1_HUMAN

Porcine

1014

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Undiluted original Porcine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Assay Type: Sandwich. Detection Range: 1.56ng/ml-50ng/ml. Sensitivity: 0.1ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, NOT for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of PARP (hereafter termed "analyte") in undiluted original Porcine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

156523968

NP_001609.2

NM_001618.3

P09874

113,084 Da

BER Complex Pathway (413429); BER Complex Pathway (468377); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Caspase Cascade In Apoptosis Pathway (137974); Corticotropin-releasing Hormone Pathway (920957); Disease Pathway (530764); Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway (645266); FAS Pathway And Stress Induction Of HSP Regulation (198894); Gene Expression Pathway (105937)

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

PARP1: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK. Protein type: DNA repair, damage; Transferase; Nucleolus; Nuclear receptor co-regulator; Nuclear envelope; EC 2.4.2.30. Chromosomal Location of Human Ortholog: 1q41-q42. Cellular Component: nucleoplasm; transcription factor complex; membrane; nucleolus; nuclear envelope; nucleus. Molecular Function: identical protein binding; protein binding; enzyme binding; DNA binding; zinc ion binding; protein N-terminus binding; transcription factor binding; protein kinase binding; NAD binding; NAD+ ADP-ribosyltransferase activity. Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; macrophage differentiation; transcription, DNA-dependent; DNA damage response, detection of DNA damage; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein autoprocessing; protein amino acid ADP-ribosylation; cellular response to insulin stimulus; base-excision repair; transforming growth factor beta receptor signaling pathway; double-strand break repair; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of growth rate; telomere maintenance

48-Strip-Wells

435 USD

96-Strip-Wells

600