Human R-Spondin 1 ELISA Kit | MyBioSource MBS050315 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human R-Spondin 1 ELISA Kit

catalog :

MBS050315

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS050315

products type :

ELISA Kit

products full name :

Human R-Spondin 1 ELISA Kit

products short name :

R-Spondin 1

other names :

R-spondin-1 isoform 1; R-spondin-1; R-spondin-1; R-spondin homolog; roof plate-specific spondin-1; R-spondin 1; Roof plate-specific spondin-1

products gene name :

RSPO1

other gene names :

RSPO1; RSPO1; RSPO; CRISTIN3; hRspo1

uniprot entry name :

RSPO1_HUMAN

reactivity :

Human

sequence length :

263

specificity :

No significant cross-reactivity or interference between Human RSPO1 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated RSPO1 concentrations in Human serum, plasma and other body fluids. Using Purified Human RSPO1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RSPO1 and RSPO1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RSPO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated RSPO1 concentrations in Human serum, plasma and other body fluids. Using Purified Human RSPO1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RSPO1 and RSPO1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RSPO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

84490388

ncbi acc num :

NP_001033722.1

ncbi gb acc num :

NM_001038633.3

uniprot acc num :

Q2MKA7

ncbi mol weight :

28,959 Da

ncbi pathways :

Disease Pathway (530764); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1016209); Signal Transduction Pathway (477114); Signaling By WNT In Cancer Pathway (1016191); Signaling By Wnt Pathway (106510); TCF Dependent Signaling In Response To WNT Pathway (980475); Wnt Signaling Network Pathway (137962); XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway (1016210); Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway (1016208); Regulation Of FZD By Ubiquitination Pathway (980483)

ncbi summary :

This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

uniprot summary :

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide; Cell adhesion. Chromosomal Location of Human Ortholog: 1p34.3. Cellular Component: extracellular space; extracellular region; nucleus. Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding. Biological Process: regulation of gene expression; regulation of receptor internalization; positive regulation of protein amino acid phosphorylation; Wnt receptor signaling pathway through beta-catenin; male meiosis; positive regulation of Wnt receptor signaling pathway. Disease: Palmoplantar Hyperkeratosis With Squamous Cell Carcinoma Of Skin And 46,xx Sex Reversal

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage