catalog number :
MBS050141
products type :
ELISA Kit
products full name :
Rat Spondin 1 ELISA Kit
products short name :
Spondin 1
products gene name :
SPON1
specificity :
No significant cross-reactivity or interference between Rat SPON1 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SPON1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat SPON1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPON1 and SPON1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPON1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SPON1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat SPON1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPON1 and SPON1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPON1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.