Rat Excitatory Amino Acid Transporter 1 ELISA Kit | MyBioSource MBS050004 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Excitatory Amino Acid Transporter 1 ELISA Kit

catalog :

MBS050004

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS050004

products type :

ELISA Kit

products full name :

Rat Excitatory Amino Acid Transporter 1 ELISA Kit

products short name :

Excitatory Amino Acid Transporter 1

other names :

excitatory amino acid transporter 1 isoform 4; Excitatory amino acid transporter 1; excitatory amino acid transporter 1; GLAST-1; solute carrier family 1 member 3; sodium-dependent glutamate/aspartate transporter 1; solute carrier family 1 (glial high affinity glutamate transporter), member 3; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3

products gene name :

EAAT1

other gene names :

SLC1A3; SLC1A3; EA6; EAAT1; GLAST; GLAST1; EAAT1; GLAST; GLAST1; GLAST-1

uniprot entry name :

EAA1_HUMAN

reactivity :

Rat

sequence length :

496

specificity :

No significant cross-reactivity or interference between Rat EAAT1 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated EAAT1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat EAAT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EAAT1 and EAAT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EAAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated EAAT1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat EAAT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EAAT1 and EAAT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EAAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

584277111

ncbi acc num :

NP_001276868.1

ncbi gb acc num :

NM_001289939.1

uniprot acc num :

P43003

ncbi mol weight :

59,572 Da

ncbi pathways :

Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway (106533); Glutamatergic Synapse Pathway (213818); Glutamatergic Synapse Pathway (213811); Neuronal System Pathway (106513); Neurotransmitter Uptake And Metabolism In Glial Cells Pathway (106532); SIDS Susceptibility Pathways (198901); SLC-mediated Transmembrane Transport Pathway (119558); Synaptic Vesicle Pathway (672457); Transmembrane Transport Of Small Molecules Pathway (106572); Transmission Across Chemical Synapses Pathway (106516)

ncbi summary :

This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]

uniprot summary :

SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transporter; Mitochondrial; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 5p13. Cellular Component: cell surface; cell projection; membrane; cell soma; fibril; integral to membrane; plasma membrane. Molecular Function: glutamate binding; high-affinity glutamate transmembrane transporter activity; sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity. Biological Process: response to drug; response to light stimulus; cranial nerve development; auditory behavior; glutamate biosynthetic process; positive regulation of synaptic transmission; response to antibiotic; L-glutamate import; synaptic transmission; sensory perception of sound; neuron morphogenesis during differentiation; gamma-aminobutyric acid biosynthetic process; response to wounding; neurotransmitter uptake; ion transport; neuromuscular process controlling balance; transmembrane transport. Disease: Episodic Ataxia, Type 6

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage