Rat Uroplakin 3A ELISA Kit | MyBioSource MBS049186 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Uroplakin 3A ELISA Kit

catalog :

MBS049186

quantity :

48-Strip-Wells

price :

470 USD

product information

catalog number :

MBS049186

products type :

ELISA Kit

products full name :

Rat Uroplakin 3A ELISA Kit

products short name :

[Uroplakin 3A]

other names :

[uroplakin-3a isoform 2; Uroplakin-3a; uroplakin-3a; uroplakin 3; uroplakin III; uroplakin 3A; Uroplakin III]

products gene name :

[UPK3A]

other gene names :

[UPK3A; UPK3A; UP3A; UPK3; UPIII; UPIIIA; UPK3; UP3a; UPIII]

uniprot entry name :

UPK3A_HUMAN

reactivity :

Rat

sequence length :

166

specificity :

No significant cross-reactivity or interference between Rat UPK3A and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.5 ng/ml - 16 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated UPK3A concentrations in Rat serum, plasma and other body fluids. Using Purified Rat UPK3A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add UPK3A and UPK3A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UPK3A in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated UPK3A concentrations in Rat serum, plasma and other body fluids. Using Purified Rat UPK3A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add UPK3A and UPK3A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UPK3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

266456217

ncbi acc num :

NP_001161046.1

ncbi gb acc num :

NM_001167574.1

uniprot acc num :

O75631

ncbi mol weight :

30,670 Da

ncbi summary :

This gene encodes a member of the uroplakin family, a group of transmembrane proteins that form complexes on the apical surface of the bladder epithelium. Mutations in this gene may be associated with renal adysplasia. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]

uniprot summary :

UPK3A: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence. Defects in UPK3A are a cause of renal adysplasia (RADYS); also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy. Belongs to the uroplakin-3 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 22q13.31. Cellular Component: endoplasmic reticulum membrane; apical plasma membrane; integral to membrane. Biological Process: epithelial cell differentiation; urinary bladder development; water transport; urea transport; cell morphogenesis; sodium ion homeostasis; kidney development; potassium ion homeostasis. Disease: Renal Hypodysplasia/aplasia 1

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

680

size3 :

5x96-Strip-Wells

price3 :

3100

size4 :

10x96-Strip-Wells

price4 :

6095