catalog number :
MBS047619
products type :
ELISA Kit
products full name :
Camel Semenogelin I ELISA Kit
products short name :
[Semenogelin I]
other names :
[semenogelin; Semenogelin-1; semenogelin-1; cancer/testis antigen 103; semen coagulating protein; semenogelin I; Semenogelin I]
products gene name :
[SEMG1]
other gene names :
[SEMG1; SEMG1; SGI; SEMG; CT103; dJ172H20.2; SEMG; SGI]
uniprot entry name :
SEMG1_HUMAN
specificity :
No significant cross-reactivity or interference between Camel SEMG1 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SEMG1 concentrations in Camel serum, plasma and other body fluids. Using Purified Camel SEMG1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SEMG1 and SEMG1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SEMG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
AAB59506.1
ncbi mol weight :
52,131 Da
ncbi pathways :
Amyloids Pathway (366238); Disease Pathway (530764)
ncbi summary :
The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. The prostate-specific antigen (PSA) protease processes this protein into smaller peptides, with each possibly having a separate function. The proteolysis process breaks down the gel matrix and allows the spermatozoa to move more freely. [provided by RefSeq, Jul 2009]
uniprot summary :
SEMG1: Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA. Belongs to the semenogelin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Cancer Testis Antigen (CTA). Chromosomal Location of Human Ortholog: 20q12-q13.2. Cellular Component: extracellular space; extracellular region; nucleus; secretory granule. Molecular Function: protein binding; structural molecule activity. Biological Process: insemination
size4 :
10x96-Strip-Wells