Human Leukotriene D4 ELISA Kit | MyBioSource MBS047305 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Leukotriene D4 ELISA Kit

catalog :

MBS047305

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS047305

products type :

ELISA Kit

products full name :

Human Leukotriene D4 ELISA Kit

products short name :

Leukotriene D4

other names :

leukotriene D4 receptor; Cysteinyl leukotriene receptor 1; cysteinyl leukotriene receptor 1; LTD4 receptor; cysteinyl leukotriene D4 receptor; cysteinyl leukotriene 1 receptor short isoform; cysteinyl leukotriene receptor 1; Cysteinyl leukotriene D4 receptor

products gene name :

LT-D4

other gene names :

Cysltr1; Cysltr1; Cyslt1; CysLT1R; BB147369; Cyslt1; Cyslt1r; CysLTR1

uniprot entry name :

CLTR1_MOUSE

reactivity :

Human

sequence length :

352

specificity :

No significant cross-reactivity or interference between Human LT-D4 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.625ng/ml-20ng/ml. Sensitivity: 0.1ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated LT-D4 concentrations in Human serum, plasma and other body fluids. Using Purified Human LT-D4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LT-D4 and LT-D4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LT-D4 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated LT-D4 concentrations in Human serum, plasma and other body fluids. Using Purified Human LT-D4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LT-D4 and LT-D4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LT-D4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

8118595

ncbi acc num :

AAF73047.1

uniprot acc num :

Q99JA4

ncbi mol weight :

40,715 Da

ncbi pathways :

Calcium Signaling Pathway (83247); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); Eicosanoid Ligand-binding Receptors Pathway (1000957); G Alpha (q) Signalling Events Pathway (1000975); GPCR Downstream Signaling Pathway (1000971); GPCR Ligand Binding Pathway (1000941); GPCRs, Class A Rhodopsin-like Pathway (198308); GPCRs, Other Pathway (198282); Gastrin-CREB Signalling Pathway Via PKC And MAPK (970815)

uniprot summary :

CYSLTR1: Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 LTE4 = LTC4 LTB4. Belongs to the G-protein coupled receptor 1 family. Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR. Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; signal transducer activity; leukotriene receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; cell surface receptor linked signal transduction; calcium ion transport; positive regulation of vasoconstriction; signal transduction; chemotaxis; inflammatory response

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage