ELISA/assay

Mouse Oxidized Lowdensity Lipoprotein ELISA Kit

MBS046663

96 Strip Wells

600 USD

ELISA Kit

Mouse Oxidized Lowdensity Lipoprotein ELISA Kit

Oxidized Lowdensity Lipoprotein

oxidized low-density lipoprotein receptor 1 isoform 1; Oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1; hLOX-1; ox LDL receptor 1; lectin-type oxidized LDL receptor 1; scavenger receptor class E, member 1; C-type lectin domain family 8 member A; oxidized low-density lipoprotein receptor 1, soluble form; oxidized low density lipoprotein (lectin-like) receptor 1; C-type lectin domain family 8 member A; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1; Lectin-type oxidized LDL receptor 1

OXLDL

OLR1; OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1; CLEC8A; LOX1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1

OLR1_HUMAN

Mouse

273

No significant cross-reactivity or interference between Mouse OXLDL and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Detection Range: The detection range of this kit is 1.25 ug/ml – 40 ug/ml.

Sensitivity: The sensitivity of this kit is 0.1 ug/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate OXLDL concentrations in Mouse serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications!

4505501

NP_002534.1

NM_002543.3

P78380

30,959 Da

Cell Surface Interactions At The Vascular Wall Pathway 106062!!Hemostasis Pathway 106028!!PPAR Signaling Pathway 83042!!PPAR Signaling Pathway 450!!Phagosome Pathway 153910!!Phagosome Pathway 153859

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Function: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. Ref.1 Ref.13 Ref.14. Subunit structure: Homodimer; disulfide-linked. May form a hexamer composed of 3 homodimers. Interacts with HSP70. Ref.3 Ref.19 Ref.27 Ref.28. Subcellular location: Cell membrane; Lipid-anchor. Cell membrane; Single-pass type II membrane protein. Membrane raft. Secreted. Note: A secreted form also exists. Localization to membrane rafts requires palmitoylation. Ref.24 Ref.26. Tissue specificity: Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level. Ref.1 Ref.4 Ref.14. Induction: By inflammatory cytokines such as TNF, IFNG/IFN-gamma, IL6/interleukin-6 and by pathological conditions such as hyperlipidemia, hypertension and diabetes mellitus. Up-regulated in atherosclerotic lesions, by oxLDL, reactive oxygen species and fluid shear stress, suggesting that it may participate in amplification of oxLDL-induced vascular dysfunction. Ref.4 Ref.16. Domain: The cytoplasmic region is required for subcellular sorting on the cell surface. Ref.3 Ref.24The C-type lectin domain mediates the recognition and binding of oxLDL. Ref.3 Ref.24. Post-translational modification: The intrachain disulfide-bonds prevent N-glycosylation at some sites.N-glycosylated. Ref.3 Ref.25. Involvement in disease: Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility. Ref.15 Ref.18 Ref.20 Ref.21 Ref.23OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (Ref.14, Ref.17 and Ref.22), variations in OLR1 modify the risk of AD, while according to other (Ref.19 and Ref.20) they do not. Ref.15 Ref.18 Ref.20 Ref.21 Ref.23. Sequence similarities: Contains 1 C-type lectin domain.

96 Strip Wells

600 USD