Rat Myeloperoxidase ELISA Kit | MyBioSource MBS046496 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Myeloperoxidase ELISA Kit

catalog :

MBS046496

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS046496

products type :

ELISA Kit

products full name :

Rat Myeloperoxidase ELISA Kit

products short name :

Myeloperoxidase

other names :

myeloperoxidase; Myeloperoxidase; myeloperoxidase; myeloperoxidase

products gene name :

MPO

other gene names :

MPO; MPO; MPO

uniprot entry name :

PERM_HUMAN

reactivity :

Rat

specificity :

No significant cross-reactivity or interference between Rat MPO and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 25 U/L - 800 U/L. Sensitivity: 2.0 U/L.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MPO concentrations in Rat serum, plasma and other body fluids. Using Purified Rat MPO antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MPO and MPO antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MPO in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MPO concentrations in Rat serum, plasma and other body fluids. Using Purified Rat MPO antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MPO and MPO antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

188658

ncbi acc num :

AAA59863.1

uniprot acc num :

P05164

ncbi mol weight :

83,869 Da

ncbi pathways :

C-MYB Transcription Factor Network Pathway (138073); Folate Metabolism Pathway (198833); IL23-mediated Signaling Events Pathway (138000); Phagosome Pathway (153910); Phagosome Pathway (153859); Selenium Pathway (198825); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); Amb2 Integrin Signaling Pathway (137945)

ncbi summary :

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]

uniprot summary :

MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; EC 1.11.2.2. Chromosomal Location of Human Ortholog: 17q23.1. Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule. Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding. Biological Process: response to food; respiratory burst during defense response; removal of superoxide radicals; hydrogen peroxide catabolic process; response to mechanical stimulus; defense response; response to lipopolysaccharide; response to yeast; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging. Disease: Myeloperoxidase Deficiency; Alzheimer Disease

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage