ELISA/assay

Canine Macrophage Migration Inhibitory Factor ELISA Kit

MBS046444

48-Strip-Wells

435 USD

ELISA Kit

Canine Macrophage Migration Inhibitory Factor ELISA Kit

Macrophage Migration Inhibitory Factor

macrophage migration inhibitory factor; Macrophage migration inhibitory factor; macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase

MIF

MIF; MIF; GIF; GLIF; MMIF; GLIF; MMIF; MIF; GIF

MIF_HUMAN

Canine

No significant cross-reactivity or interference between Canine MIF and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MIF concentrations in Canine serum, plasma and other body fluids. Using Purified Canine MIF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MIF and MIF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MIF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MIF concentrations in Canine serum, plasma and other body fluids. Using Purified Canine MIF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MIF and MIF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MIF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4505185

NP_002406.1

NM_002415.1

P14174

12,476 Da

Adipogenesis Pathway (198832); Phenylalanine Metabolism Pathway (82960); Phenylalanine Metabolism Pathway (327); Spinal Cord Injury Pathway (739007); Tyrosine Metabolism Pathway (82959); Tyrosine Metabolism Pathway (325)

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase. Protein type: Cytokine; Apoptosis; Isomerase; EC 5.3.2.1; EC 5.3.3.12; Amino Acid Metabolism - phenylalanine; Cell development/differentiation; Amino Acid Metabolism - tyrosine. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; vesicle. Molecular Function: phenylpyruvate tautomerase activity; protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; dopachrome isomerase activity; receptor binding; chemoattractant activity. Biological Process: DNA damage response, signal transduction by p53 class mediator; regulation of macrophage activation; positive regulation of lipopolysaccharide-mediated signaling pathway; carboxylic acid metabolic process; cell aging; negative regulation of cellular protein metabolic process; prostaglandin biosynthetic process; positive regulation of peptidyl-serine phosphorylation; positive regulation of MAP kinase activity; cell proliferation; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of fibroblast proliferation; negative regulation of myeloid cell apoptosis; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of mature B cell apoptosis; cell surface receptor linked signal transduction; positive chemotaxis; positive regulation of B cell proliferation; innate immune response; inflammatory response; positive regulation of phosphorylation; negative regulation of apoptosis; positive regulation of cytokine secretion. Disease: Rheumatoid Arthritis, Systemic Juvenile

48-Strip-Wells

435 USD

96-Strip-Wells

600