catalog number :
MBS046218
products type :
ELISA Kit
products full name :
Bovine Prostaglandin I2 ELISA Kit
products short name :
Prostaglandin I2
products gene name :
PGI2
specificity :
No significant cross-reactivity or interference between Bovine PGI2 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 15.6 pg/ml - 500 pg/ml. Sensitivity: 2.0 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PGI2 concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine PGI2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PGI2 and PGI2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PGI2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PGI2 concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine PGI2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PGI2 and PGI2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PGI2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.