ELISA/assay

Human Fructose-1,6-Bisphosphate Aldolase ELISA Kit

MBS046088

48-Strip-Wells

435 USD

ELISA Kit

Human Fructose-1,6-Bisphosphate Aldolase ELISA Kit

Fructose-1,6-Bisphosphate Aldolase

Fructose-bisphosphate aldolase class 2; fructose-bisphosphate aldolase, class II

FDA

fbaA

Human

359

No significant cross-reactivity or interference between Human FDA and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25U/L-200U/L. Sensitivity: 1.0U/L.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FDA concentrations in Human serum, plasma and other body fluids. Using Purified Human FDA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FDA and FDA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FDA in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FDA concentrations in Human serum, plasma and other body fluids. Using Purified Human FDA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FDA and FDA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FDA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

81175127

P0AB71.2

Biosynthesis Of Amino Acids Pathway (790553); Biosynthesis Of Amino Acids Pathway (795174); Biosynthesis Of Secondary Metabolites Pathway (148731); Carbon Metabolism Pathway (815454); Carbon Metabolism Pathway (817567); Fructose And Mannose Metabolism Pathway (6744); Fructose And Mannose Metabolism Pathway (291); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (404587); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (404585)

Binds TrxA (Kumar, 2004). kbaY, gatY, fbaA and ydjI are paralogs. [More information is available at EcoGene: EG10282]. The amino acid sequence of the Escherichia coli Class II fructose-1,6-bisphosphate aldolase inferred from the DNA sequence of the fda gene is the first primary structure of a Class II aldolase to be established. [More information is available at EcoCyc: EG10282].

48-Strip-Wells

435 USD

96-Strip-Wells

600