Porcine Gastric Inhibitory Polypeptide ELISA Kit | MyBioSource MBS045417 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine Gastric Inhibitory Polypeptide ELISA Kit

catalog :

MBS045417

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS045417

products type :

ELISA Kit

products full name :

Porcine Gastric Inhibitory Polypeptide ELISA Kit

products short name :

[Gastric Inhibitory Polypeptide]

other names :

[gastric inhibitory polypeptide preproprotein; Gastric inhibitory polypeptide; gastric inhibitory polypeptide; incretin hormone; glucose-dependent insulinotropic polypeptide; gastric inhibitory polypeptide; Glucose-dependent insulinotropic polypeptide; Incretin hormone]

products gene name :

[GIP]

other gene names :

[GIP; GIP; GIP]

uniprot entry name :

GIP_HUMAN

reactivity :

Porcine

specificity :

No significant cross-reactivity or interference between Porcine GIP and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 50 pg/ml - 1600 pg/ml. Sensitivity: 10 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GIP concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine GIP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GIP and GIP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GIP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GIP concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine GIP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GIP and GIP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4758436

ncbi acc num :

NP_004114.1

ncbi gb acc num :

NM_004123.2

uniprot acc num :

P09681

ncbi mol weight :

17,108 Da

ncbi pathways :

Class B/2 (Secretin Family Receptors) Pathway (106378); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Glucagon-type Ligand Receptors Pathway (106380); Incretin Synthesis, Secretion, And Inactivation Pathway (187170); Insulin Secretion Pathway (777534); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Signal Transduction Pathway (477114)

ncbi summary :

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]

uniprot summary :

GIP: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Belongs to the glucagon family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q21.3-q22. Cellular Component: extracellular space; cell soma; endoplasmic reticulum lumen; extracellular region. Molecular Function: hormone activity. Biological Process: response to drug; response to peptide hormone stimulus; adult locomotory behavior; positive regulation of insulin secretion; female pregnancy; sensory perception of pain; response to lipid; signal transduction; response to amino acid stimulus; response to organic cyclic substance; endocrine pancreas development; memory; response to starvation; response to selenium ion; cellular protein metabolic process; response to glucose stimulus; response to axon injury; regulation of insulin secretion

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

680

size3 :

5x96-Strip-Wells

price3 :

3100

size4 :

10x96-Strip-Wells

price4 :

6095

more info or order :

MyBioSource product webpage