ELISA/assay

Porcine Gamma Glutamyltransferase 1 ELISA Kit

MBS045056

48-Strip-Wells

435 USD

ELISA Kit

Porcine Gamma Glutamyltransferase 1 ELISA Kit

Gamma Glutamyltransferase 1

GGT1; Gamma-glutamyltranspeptidase 1; gamma-glutamyltranspeptidase 1; glutamyl transpeptidase; glutathione hydrolase 1; leukotriene-C4 hydrolase; gamma-glutamyl transpeptidase; gamma-glutamyltransferase 1; Gamma-glutamyltransferase 1; Glutathione hydrolase 1 (EC:3.4.19.13); Leukotriene-C4 hydrolase (EC:3.4.19.14); CD_antigen: CD224Cleaved into the following 2 chains:Gamma-glutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain

GGT1

GGT1; GGT1; GGT; GTG; CD224; GGT 1; D22S672; D22S732; GGT; GGT 1

GGT1_HUMAN

Porcine

No significant cross-reactivity or interference between Porcine GGT1 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 U/L - 200 U/L. Sensitivity: 1.0 U/L.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GGT1 concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine GGT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GGT1 and GGT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GGT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GGT1 concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine GGT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GGT1 and GGT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GGT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

47678519

CAG30380.1

P19440

61,410 Da

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Biological Oxidations Pathway (105698); Cyanoamino Acid Metabolism Pathway (82970); Cyanoamino Acid Metabolism Pathway (339); Eicosanoid Synthesis Pathway (198888); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824)

The enzyme encoded by this gene catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized and some of which may represent pseudogenes. This gene is classified as type I gamma-glutamyltransferase. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Oct 2008]

GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter. Protein type: EC 2.3.2.2; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - selenoamino acid; Other Amino Acids Metabolism - glutathione; Other Amino Acids Metabolism - cyanoamino acid; EC 3.4.19.13; Lipid Metabolism - arachidonic acid; Transferase; Membrane protein, integral; EC 3.4.19.14. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: anchored to external side of plasma membrane; extracellular space; plasma membrane; integral to membrane. Molecular Function: protein binding; gamma-glutamyltransferase activity. Biological Process: amino acid metabolic process; glutamate metabolic process; regulation of immune system process; cysteine biosynthetic process; leukotriene metabolic process; proteolysis; leukotriene biosynthetic process; glutathione metabolic process; xenobiotic metabolic process; glutathione catabolic process; regulation of inflammatory response; glutathione biosynthetic process; arachidonic acid metabolic process; zymogen activation; spermatogenesis. Disease: Glutathionuria

48-Strip-Wells

435 USD

96-Strip-Wells

600