Human Matrix Metalloproteinase 8 ELISA Kit | MyBioSource MBS044946 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Matrix Metalloproteinase 8 ELISA Kit

catalog :

MBS044946

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS044946

products type :

ELISA Kit

products full name :

Human Matrix Metalloproteinase 8 ELISA Kit

products short name :

Matrix Metalloproteinase 8

other names :

matrix metalloproteinase 8 (neutrophil collagenase); Neutrophil collagenase; neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase); matrix metallopeptidase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP-8; PMNL collagenase

products gene name :

MMP-8

other gene names :

MMP8; MMP8; HNC; CLG1; MMP-8; PMNL-CL; CLG1; MMP-8; PMNL-CL

uniprot entry name :

MMP8_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human MMP-8 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MMP-8 concentrations in Human serum, plasma and other body fluids. Using Purified Human MMP-8 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MMP-8 and MMP-8 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MMP-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MMP-8 concentrations in Human serum, plasma and other body fluids. Using Purified Human MMP-8 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MMP-8 and MMP-8 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MMP-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

71648770

ncbi acc num :

AAZ38714.1

uniprot acc num :

P22894

ncbi mol weight :

53,412 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of Collagen Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Matrix Metalloproteinases Pathway (198900)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

uniprot summary :

MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family. Protein type: Secreted, signal peptide; Secreted; EC 3.4.24.34; Protease. Chromosomal Location of Human Ortholog: 11q22.3. Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: zinc ion binding; metalloendopeptidase activity; serine-type endopeptidase activity; calcium ion binding. Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage