Horse Cathepsin K ELISA Kit | MyBioSource MBS044439 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Horse Cathepsin K ELISA Kit

catalog :

MBS044439

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS044439

products type :

ELISA Kit

products full name :

Horse Cathepsin K ELISA Kit

products short name :

Cathepsin K

other names :

cathepsin K preproprotein; Cathepsin K; cathepsin K; cathepsin O; cathepsin X; cathepsin O1; cathepsin O2; cathepsin K; Cathepsin O; Cathepsin O2; Cathepsin X

products gene name :

CTSK

other gene names :

CTSK; CTSK; CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2; CTSO; CTSO2

uniprot entry name :

CATK_HUMAN

reactivity :

Horse

specificity :

No significant cross-reactivity or interference between Horse CTSK and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CTSK concentrations in Horse serum, plasma and other body fluids. Using Purified Horse CTSK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CTSK and CTSK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CTSK in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CTSK concentrations in Horse serum, plasma and other body fluids. Using Purified Horse CTSK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CTSK and CTSK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CTSK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4503151

ncbi acc num :

NP_000387.1

ncbi gb acc num :

NM_000396.3

uniprot acc num :

P43235

ncbi mol weight :

36,966 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Adaptive Immune System Pathway (366160); Degradation Of Collagen Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Immune System Pathway (106386); Innate Immune System Pathway (106387); Lysosome Pathway (99052); Lysosome Pathway (96865); MHC Class II Antigen Presentation Pathway (645290)

ncbi summary :

The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]

uniprot summary :

CTSK: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Defects in CTSK are the cause of pycnodysostosis (PKND). PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. Belongs to the peptidase C1 family. Protein type: EC 3.4.22.38; Protease. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular space; extracellular region. Molecular Function: collagen binding; proteoglycan binding; protein binding; fibronectin binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity. Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; intramembranous ossification; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; proteolysis; bone resorption. Disease: Pycnodysostosis

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage