Mouse Keratin Growth Factor ELISA Kit | MyBioSource MBS044418 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Keratin Growth Factor ELISA Kit

catalog :

MBS044418

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS044418

products type :

ELISA Kit

products full name :

Mouse Keratin Growth Factor ELISA Kit

products short name :

Keratin Growth Factor

other names :

keratin, type II cytoskeletal 8 isoform 2; Keratin, type II cytoskeletal 8; keratin, type II cytoskeletal 8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8; keratin 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8

products gene name :

KGF

other gene names :

KRT8; KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2; CYK8; CK-8; K8

uniprot entry name :

K2C8_HUMAN

reactivity :

Mouse

sequence length :

483

specificity :

This kit recognizes recombinant and natural Mouse KGF, no significant cross-reactivity or interference was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Detection Range: 6.25 pg/ml ~ 200 pg/ml. Sensitivity: 1.0 pg/ml.

other info2 :

Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Mouse KGF in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Mouse KGF concentration. The concentration of Mouse KGF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) are less than 15%. Inter-assay Precision: Inter-assay CV (%) are less than 15%.

products description :

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

ncbi gi num :

4504919

ncbi acc num :

NP_002264.1

ncbi gb acc num :

NM_002273.3

uniprot acc num :

P05787

ncbi mol weight :

53,704 Da

ncbi pathways :

EGFR1 Signaling Pathway (198782); Signaling Mediated By P38-alpha And P38-beta Pathway (138012)

ncbi summary :

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

uniprot summary :

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: nucleoplasm; dystrophin-associated glycoprotein complex; intermediate filament cytoskeleton; costamere; nuclear matrix; cytoplasm; keratin filament; intermediate filament; intercellular junction; nucleus; Z disc; sarcolemma. Molecular Function: protein binding; protein complex binding; structural molecule activity. Biological Process: viral reproduction; tumor necrosis factor-mediated signaling pathway; sarcomere organization; response to other organism; response to hydrostatic pressure. Disease: Cirrhosis, Familial

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage