catalog number :
MBS044339
products type :
ELISA Kit
products full name :
Chicken Thyrotropin Releasing Hormone ELISA Kit
products short name :
Thyrotropin Releasing Hormone
other names :
thyrotropin releasing hormone preproprotein; Pro-thyrotropin-releasing hormone; thyrotropin releasing hormone; protirelin; prothyroliberin; TSH-releasing factor; thyrotropin-releasing factor; thyrotropin-releasing hormone; ProthyroliberinCleaved into the following chain:Thyrotropin-releasing hormone; TRH; Alternative name(s):; Protirelin; TSH-releasing factor; Thyroliberin; Thyrotropin-releasing factor
other gene names :
TRH; TRH; TRF; Pro-TRH; TRH; TRF
uniprot entry name :
TRH_HUMAN
specificity :
No significant cross-reactivity or interference between Chicken TRH and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.625 ?IU/ml - 20 ?IU/ml. Sensitivity: 0.1 ?IU/ml.
other info2 :
Intended Uses: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate TRH concentrations in Chicken serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
products description :
Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate TRH concentrations in Chicken serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications!
ncbi acc num :
NP_009048.1
ncbi gb acc num :
NM_007117.4
ncbi mol weight :
27,404 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)
ncbi summary :
This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. [provided by RefSeq, May 2013]
uniprot summary :
TRH: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. May promote hair shaft elongation, prolonge the hair cycle growth phase (anagen) and antagonized its termination by TGFB2. May also increase proliferation and inhibited apoptosis of hair matrix keratinocytes. Belongs to the TRH family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3q13.3-q21. Cellular Component: plasma membrane; extracellular region; nucleus; secretory granule. Molecular Function: neuropeptide hormone activity; thyrotropin-releasing hormone activity. Biological Process: hormone-mediated signaling; negative regulation of glutamate secretion; eating behavior; positive regulation of insulin secretion; histamine metabolic process; signal transduction; adult walking behavior; response to ethanol; cell-cell signaling; response to hypoxia; response to glucose stimulus; response to cold; response to corticosterone stimulus; positive regulation of gamma-aminobutyric acid secretion. Disease: Thyrotropin-releasing Hormone Deficiency