ELISA/assay

Mouse Interleukin 31 ELISA Kit

MBS044259

48-Strip-Wells

435 USD

ELISA Kit

Mouse Interleukin 31 ELISA Kit

Interleukin 31

interleukin-31; Interleukin-31; interleukin-31; interleukin 31

IL-31

IL31; IL31; IL-31; IL-31

IL31_HUMAN

Mouse

No significant cross-reactivity or interference between Mouse IL-31 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12pg/ml-100pg/ml. Sensitivity: 1.0pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-31 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL-31 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-31 and IL-31 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-31 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-31 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL-31 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-31 and IL-31 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-31 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

62122911

NP_001014358.1

NM_001014336.1

Q6EBC2

18,205 Da

IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM, Mar 2008]

IL31: Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. Protein type: Cytokine; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q24.31. Cellular Component: extracellular space. Molecular Function: cytokine activity. Biological Process: immune system process

48-Strip-Wells

435 USD

96-Strip-Wells

600