Canine Proliferating Cell Nuclear Antigen ELISA Kit | MyBioSource MBS044210 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Proliferating Cell Nuclear Antigen ELISA Kit

catalog :

MBS044210

quantity :

48-Strip-Wells

price :

470 USD

product information

catalog number :

MBS044210

products type :

ELISA Kit

products full name :

Canine Proliferating Cell Nuclear Antigen ELISA Kit

products short name :

[Proliferating Cell Nuclear Antigen]

other names :

[PCNA; Proliferating cell nuclear antigen; proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein; proliferating cell nuclear antigen]

products gene name :

[PCNA]

other gene names :

[PCNA; PCNA]

uniprot entry name :

Q6FHF5_HUMAN

reactivity :

Canine

specificity :

No significant cross-reactivity or interference between this analyte and analogues is observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Undiluted original Canine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Assay Type: Sandwich. Detection Range: 0.5 ng/ml - 16 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, NOT for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of PCNA (hereafter termed "analyte") in undiluted original Canine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

ncbi gi num :

49456555

ncbi acc num :

CAG46598.1

uniprot acc num :

Q6FHF5

ncbi mol weight :

28,706 Da

ncbi pathways :

BARD1 Signaling Events Pathway (137959); BRCA1-associated Genome Surveillance Complex (BASC) Pathway (413428); Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463)

ncbi summary :

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

uniprot summary :

PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase s processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3 - 5 exonuclease and 3 -phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family. Protein type: DNA replication; Cell cycle regulation. Chromosomal Location of Human Ortholog: 20pter-p12. Cellular Component: nucleoplasm; centrosome; PCNA complex; nuclear replication fork; cytoplasm; DNA replication factor C complex; nucleus. Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; purine-specific mismatch base pair DNA N-glycosylase activity; MutLalpha complex binding; dinucleotide insertion or deletion binding; receptor tyrosine kinase binding. Biological Process: mismatch repair; positive regulation of deoxyribonuclease activity; heart development; DNA strand elongation during DNA replication; DNA repair; response to lipid; leading strand elongation; G1/S-specific transcription in mitotic cell cycle; telomere maintenance via semi-conservative replication; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; nucleotide-excision repair; base-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; telomere maintenance; G1/S transition of mitotic cell cycle; regulation of DNA replication. Disease: Ataxia-telangiectasia-like Disorder 2

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

680

size3 :

5x96-Strip-Wells

price3 :

3100

size4 :

10x96-Strip-Wells

price4 :

6095