ELISA/assay

Rat Deoxypyridinoline ELISA Kit

MBS043935

48-Strip-Wells

435 USD

ELISA Kit

Rat Deoxypyridinoline ELISA Kit

Deoxypyridinoline

Dihydropyrimidine dehydrogenase

DPD

Rat

1025

No significant cross-reactivity or interference between Rat DPD and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.5 nmol/L - 16 nmol/L. Sensitivity: 0.1 nmol/L.

Intended Uses: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate DPD concentrations in Rat serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate DPD concentrations in Rat serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications!

160332325

Q12882.2

Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); Fluoropyrimidine Activity Pathway (198778); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Pantothenate And CoA Biosynthesis Pathway (83015); Pantothenate And CoA Biosynthesis Pathway (401); Pyrimidine Catabolism Pathway (106288); Pyrimidine Degradation, Uracil = Beta-alanine, Thymine = 3-aminoisobutanoate Pathway (605866); Pyrimidine Degradation, Uracil = Beta-alanine, Thymine = 3-aminoisobutanoate Pathway (468239)

The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family. Protein type: Oxidoreductase; Xenobiotic Metabolism - drug metabolism - other enzymes; Other Amino Acids Metabolism - beta-alanine; Nucleotide Metabolism - pyrimidine; EC 1.3.1.2; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis. Chromosomal Location of Human Ortholog: 1p22. Cellular Component: cytoplasm; cytosol. Molecular Function: protein homodimerization activity; FAD binding; dihydroorotate dehydrogenase activity; metal ion binding; 4 iron, 4 sulfur cluster binding; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding. Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; UMP biosynthetic process; uracil catabolic process; thymine catabolic process; beta-alanine biosynthetic process; purine base catabolic process; pyrimidine base catabolic process; thymidine catabolic process. Disease: Dihydropyrimidine Dehydrogenase Deficiency

48-Strip-Wells

435 USD

96-Strip-Wells

600