Bovine Serum Amyloid P Component ELISA Kit | MyBioSource MBS043263 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine Serum Amyloid P Component ELISA Kit

catalog :

MBS043263

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS043263

products type :

ELISA Kit

products full name :

Bovine Serum Amyloid P Component ELISA Kit

products short name :

Serum Amyloid P Component

other names :

serum amyloid P-component; Serum amyloid P-component; serum amyloid P-component; pentaxin-related; 9.5S alpha-1-glycoprotein; amyloid P component, serum; 9.5S alpha-1-glycoprotein

products gene name :

SAP

other gene names :

APCS; APCS; SAP; PTX2; PTX2; SAP

uniprot entry name :

SAMP_HUMAN

reactivity :

Bovine

specificity :

No significant cross-reactivity or interference between Bovine SAP and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 15.6 ng/ml - 500 ng/ml. Sensitivity: 2.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SAP concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine SAP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SAP and SAP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SAP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SAP concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine SAP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SAP and SAP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4502133

ncbi acc num :

NP_001630.1

ncbi gb acc num :

NM_001639.3

uniprot acc num :

P02743

ncbi mol weight :

25,387 Da

ncbi pathways :

Amyloids Pathway (366238); Disease Pathway (530764)

ncbi summary :

The protein encoded by this gene is a glycoprotein, belonging to the pentraxin family of proteins, which has a characteristic pentameric organization. These family members have considerable sequence homology which is thought to be the result of gene duplication. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo. [provided by RefSeq, Sep 2008]

uniprot summary :

APCS: Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. Belongs to the pentaxin family. Protein type: Secreted, signal peptide; Secreted; Chaperone. Chromosomal Location of Human Ortholog: 1q21-q23. Cellular Component: extracellular matrix; extracellular space; protein complex; extracellular region; nucleus. Molecular Function: complement component C1q binding; unfolded protein binding; virion binding; calcium ion binding; carbohydrate binding. Biological Process: protein folding; negative regulation of virion penetration into host cell; acute-phase response; negative regulation of acute inflammatory response; innate immune response; chaperone-mediated protein complex assembly; negative regulation of monocyte differentiation; negative regulation of viral reproduction

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage