ELISA/assay

Human Filamin A, Alpha ELISA Kit

MBS042896

48-Strip-Wells

435 USD

ELISA Kit

Human Filamin A, Alpha ELISA Kit

Filamin A, Alpha

filamin A, alpha (actin binding protein 280), isoform CRA_c; Filamin-A; filamin-A; filamin-1; alpha-filamin; non-muscle filamin; actin binding protein 280; endothelial actin-binding protein; filamin A, alpha; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin

FLNA

FLNA; FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280; FLN; FLN1; FLN-A; ABP-280

FLNA_HUMAN

Human

2639

No significant cross-reactivity or interference between Human FLNA and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 50 pg/ml - 1600 pg/ml. Sensitivity: 10 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FLNA concentrations in Human serum, plasma and other body fluids. Using Purified Human FLNA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FLNA and FLNA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FLNA in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FLNA concentrations in Human serum, plasma and other body fluids. Using Purified Human FLNA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FLNA and FLNA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FLNA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119593152

EAW72746.1

P21333

280,739 Da

Androgen Receptor Signaling Pathway (198806); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-extracellular Matrix Interactions Pathway (160967); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); GP1b-IX-V Activation Signalling Pathway (187205); Hemostasis Pathway (106028); MAPK Signaling Pathway (198779)

The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

FLNA: a ubiquitous cytoskeletal protein that promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Plays an essential role in embryonic cell migration. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interactions with filamin A may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking. Protein type: Actin-binding; Nuclear receptor co-regulator; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: Xq28. Cellular Component: cortical cytoskeleton; focal adhesion; extracellular region; actin filament; trans-Golgi network; cytosol; actin cytoskeleton; membrane; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus. Molecular Function: Rho GTPase binding; protein homodimerization activity; Rac GTPase binding; Ral GTPase binding; transcription factor binding; actin filament binding; small GTPase binding; signal transducer activity; protein binding; protein kinase C binding; mu-type opioid receptor binding; SMAD binding; glycoprotein binding. Biological Process: actin crosslink formation; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; negative regulation of transcription factor activity; mRNA transcription from RNA polymerase II promoter; receptor clustering; dopamine receptor, adenylate cyclase inhibiting pathway; establishment of protein localization; platelet degranulation; early endosome to late endosome transport; actin cytoskeleton reorganization; cytoplasmic sequestering of protein; positive regulation of transcription factor import into nucleus; cilium biogenesis; epithelial to mesenchymal transition; blood coagulation; negative regulation of protein catabolic process. Disease: Terminal Osseous Dysplasia; Fg Syndrome 2; Frontometaphyseal Dysplasia; Cardiac Valvular Dysplasia, X-linked; Melnick-needles Syndrome; Heterotopia, Periventricular, Ehlers-danlos Variant; Intestinal Pseudoobstruction, Neuronal, Chronic Idiopathic, X-linked; Heterotopia, Periventricular, X-linked Dominant; Otopalatodigital Syndrome, Type Ii; Otopalatodigital Syndrome, Type I

48-Strip-Wells

435 USD

96-Strip-Wells

600