Fish Interleukin 1 ELISA Kit | MyBioSource MBS042749 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Fish Interleukin 1 ELISA Kit

catalog :

MBS042749

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS042749

products type :

ELISA Kit

products full name :

Fish Interleukin 1 ELISA Kit

products short name :

Interleukin 1

other names :

interleukin-1 receptor type 2 isoform 2; Interleukin-1 receptor type 2; interleukin-1 receptor type 2; IL-1R-beta; antigen CDw121b; IL-1 type II receptor; interleukin-1 receptor beta; interleukin-1 receptor type II; CD121 antigen-like family member B; type II interleukin-1 receptor, beta; interleukin 1 receptor type II variant 3; interleukin 1 receptor, type II; CD121 antigen-like family member B; CDw121b; IL-1 type II receptor; Interleukin-1 receptor beta; IL-1R-beta; Interleukin-1 receptor type II

products gene name :

IL-1

other gene names :

IL1R2; IL1R2; IL1RB; CD121b; IL1R2c; CDw121b; IL-1R-2; IL-1RT2; IL-1RT-2; IL1RB; IL-1R-2; IL-1RT-2; IL-1RT2; IL-1R-beta; mIL-1R2; mIL-1RII; sIL-1R2; sIL-1RII

uniprot entry name :

IL1R2_HUMAN

reactivity :

Fish

sequence length :

296

specificity :

No significant cross-reactivity or interference between Fish IL-1 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 15.6 pg/ml - 500 pg/ml. Sensitivity: 2.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-1 concentrations in Fish serum, plasma and other body fluids. Using Purified Fish IL-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-1 and IL-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-1 concentrations in Fish serum, plasma and other body fluids. Using Purified Fish IL-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-1 and IL-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

387527984

ncbi acc num :

NP_001248348.1

ncbi gb acc num :

NM_001261419.1

uniprot acc num :

P27930

ncbi mol weight :

45,421 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Apoptosis Modulation And Signaling Pathway (198822); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)

ncbi summary :

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Alternative splicing results in multiple transcript variants and protein isoforms. Alternative splicing produces both membrane-bound and soluble proteins. A soluble protein is also produced by proteolytic cleavage. [provided by RefSeq, May 2012]

uniprot summary :

IL-1RB: Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Belongs to the interleukin-1 receptor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 2q12. Cellular Component: extracellular region; plasma membrane; integral to membrane. Molecular Function: protein binding; interleukin-1, Type II, blocking receptor activity; interleukin-1 receptor activity. Biological Process: cytokine and chemokine mediated signaling pathway; immune response

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage