catalog number :
MBS042478
products type :
ELISA Kit
products full name :
Fish alpha-Glucosidase ELISA Kit
products short name :
alpha-Glucosidase
other names :
alpha-glucosidase; alpha-glucosidase
products gene name :
A-GLU
other gene names :
Trad_1739
specificity :
No significant cross-reactivity or interference between Fish A-GLU and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 U/ml - 100 U/ml. Sensitivity: 1.0 U/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated A-GLU concentrations in Fish serum, plasma and other body fluids. Using Purified Fish A-GLU antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add A-GLU and A-GLU antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of A-GLU in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
products description :
Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated A-GLU concentrations in Fish serum, plasma and other body fluids. Using Purified Fish A-GLU antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add A-GLU and A-GLU antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of A-GLU in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
YP_003705400.1
ncbi gb acc num :
NC_014221.1
ncbi pathways :
Galactose Metabolism Pathway (153195); Galactose Metabolism Pathway (292); Starch And Sucrose Metabolism Pathway (153227); Starch And Sucrose Metabolism Pathway (344)