ELISA/assay

Mouse Gamma Glutamyltransferase 1 ELISA Kit

MBS042078

96 Strip Wells

600 USD

ELISA Kit

Mouse Gamma Glutamyltransferase 1 ELISA Kit

Gamma Glutamyltransferase 1

GGT1; Gamma-glutamyltranspeptidase 1; gamma-glutamyltranspeptidase 1; glutamyl transpeptidase; glutathione hydrolase 1; leukotriene-C4 hydrolase; gamma-glutamyl transpeptidase; gamma-glutamyltransferase 1; Gamma-glutamyltransferase 1; Glutathione hydrolase 1 (EC:3.4.19.13); Leukotriene-C4 hydrolase (EC:3.4.19.14); CD_antigen: CD224Cleaved into the following 2 chains:Gamma-glutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain

GGT1

GGT1; GGT1; GGT; GTG; CD224; GGT 1; D22S672; D22S732; GGT; GGT 1

GGT1_HUMAN

Mouse

Store all reagents at 2-8 degree C

ELISA Type: Sandwich

47678519

CAG30380.1

P19440

61,410 Da

Arachidonic Acid Metabolism Pathway 82991!!Arachidonic Acid Metabolism Pathway 685553!!Arachidonic Acid Metabolism Pathway 366!!Biological Oxidations Pathway 105698!!Cyanoamino Acid Metabolism Pathway 82970!!Cyanoamino Acid Metabolism Pathway 339!!Eicosanoid Synthesis Pathway 198888!!Glutathione Conjugation Pathway 105727!!Glutathione Metabolism Pathway 82973!!Glutathione Metabolism Pathway 198824

The enzyme encoded by this gene catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized and some of which may represent pseudogenes. This gene is classified as type I gamma-glutamyltransferase. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Oct 2008]

Function: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Ref.7 Ref.22. Catalytic activity: A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid. Ref.22Glutathione + H2O = L-cysteinylglycine + L-glutamate. Ref.22Leukotriene C4 + H2O = leukotriene D4 + L-glutamate. Ref.22. Pathway: Sulfur metabolism; glutathione metabolism. Subunit structure: Heterodimer composed of the light and heavy chains. The active site is located in the light chain. Subcellular location: Membrane; Single-pass type II membrane protein. Tissue specificity: Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Isoform 3 is lung-specific. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein. Post-translational modification: N-glycosylated on both chains. Contains hexoses, hexosamines and sialic acid residues. Glycosylation profiles tested in kidney and liver tissues reveal the presence of tissue-specific and site-specific glycan composition, despite the overlap in composition among the N-glycans. A total of 36 glycan compositions, with 40 unique structures are observed. Up to 15 different glycans are observed at a single site, with site-specific variation in glycan composition. The difference in glycosylation profiles in the 2 tissues do not affect the enzyme activity. Ref.13 Ref.14 Ref.22. Involvement in disease: Glutathionuria (GLUTH) [MIM:231950]: Autosomal recessive disease.Note: The disease is caused by mutations affecting the gene represented in this entry. Miscellaneous: Cys-454 was thought to bind the gamma-glutamyl moiety, but mutagenesis of this residue had no effect on activity. Sequence similarities: Belongs to the gamma-glutamyltransferase family. Sequence caution: The sequence AAA35899.1 differs from that shown. Reason: Erroneous initiation.

96 Strip Wells

600 USD