Bovine Histamine ELISA Kit | MyBioSource MBS041532 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine Histamine ELISA Kit

catalog :

MBS041532

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS041532

products type :

ELISA Kit

products full name :

Bovine Histamine ELISA Kit

products short name :

Histamine

other names :

histamine H2 receptor isoform 1; Histamine H2 receptor; histamine H2 receptor; HH2R; gastric receptor 1; gastric receptor I; histamine receptor H2; Gastric receptor I

products gene name :

HIS

other gene names :

HRH2; HRH2; H2R; H2R; HH2R

uniprot entry name :

HRH2_HUMAN

reactivity :

Bovine

sequence length :

397

specificity :

No significant cross-reactivity or interference between Bovine HIS and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 1.25 ng/ml - 40 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate HIS concentrations in Bovine serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate HIS concentrations in Bovine serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications!

ncbi gi num :

197085610

ncbi acc num :

NP_001124527.1

ncbi gb acc num :

NM_001131055.1

uniprot acc num :

P25021

ncbi mol weight :

40,098 Da

ncbi pathways :

Amine Ligand-binding Receptors Pathway (106363); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastric Acid Secretion Pathway (154409); Gastric Acid Secretion Pathway (154383)

ncbi summary :

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

uniprot summary :

H2R: The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1. Chromosomal Location of Human Ortholog: 5q35.2. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: histamine receptor activity. Biological Process: gland development; G-protein signaling, coupled to cyclic nucleotide second messenger; gastrin-induced gastric acid secretion; gut development; histamine-induced gastric acid secretion; positive regulation of vasoconstriction; visual learning; regulation of synaptic plasticity; immune response; memory

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage