ELISA/assay

Human Neurotrophic Tyrosine Kinase Receptor Type 2 ELISA Kit

MBS041513

48-Strip-Wells

435 USD

ELISA Kit

Human Neurotrophic Tyrosine Kinase Receptor Type 2 ELISA Kit

Neurotrophic Tyrosine Kinase Receptor Type 2

neurotrophic tyrosine kinase receptor type 2; BDNF/NT-3 growth factors receptor; BDNF/NT-3 growth factors receptor; trkB tyrosine kinase; tyrosine kinase receptor B; tropomyosin-related kinase B; BDNF-tropomyosine receptor kinase B; neurotrophic tyrosine kinase, receptor, type 2; GP145-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B

NTRK2

NTRK2; NTRK2; TRKB; trk-B; GP145-TrkB; TRKB; Trk-B

NTRK2_HUMAN

Human

No significant cross-reactivity or interference between Human NTRK2 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NTRK2 concentrations in Human serum, plasma and other body fluids. Using Purified Human NTRK2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NTRK2 and NTRK2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NTRK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NTRK2 concentrations in Human serum, plasma and other body fluids. Using Purified Human NTRK2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NTRK2 and NTRK2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NTRK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

15217077

AAK92490.1

Q16620

91,999 Da

Activation Of TRKA Receptors Pathway (106456); Alcoholism Pathway (585563); Alcoholism Pathway (587116); BDNF Signaling Pathway (712093); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); NGF Signalling Via TRKA From The Plasma Membrane Pathway (106459); NGF-independant TRKA Activation Pathway (106458); Neurotrophic Factor-mediated Trk Receptor Signaling Pathway (137977); Neurotrophin Signaling Pathway (101143)

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternate transcriptional splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

TrkB: a receptor tyrosine kinase of the Trk family. Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 but not nerve growth factor (NGF). Involved in the development and maintenance of the nervous system. Three splice variant isoforms have been described. Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; Membrane protein, integral; TK group; Trk family. Chromosomal Location of Human Ortholog: 9q22.1. Cellular Component: cell surface; rough endoplasmic reticulum; integral to plasma membrane; dendritic spine; terminal button; excitatory synapse; perikaryon; cytosol; postsynaptic membrane; growth cone; presynaptic active zone; endosome membrane; receptor complex. Molecular Function: brain-derived neurotrophic factor receptor activity; neurotrophin binding; protein homodimerization activity; ephrin receptor binding; brain-derived neurotrophic factor binding; ATP binding. Biological Process: circadian rhythm; mechanoreceptor differentiation; retinal rod cell development; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; regulation of neurotransmitter secretion; neuron migration; regulation of dendrite development; positive regulation of synaptic transmission, glutamatergic; neuron differentiation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; oligodendrocyte differentiation; feeding behavior; negative regulation of neuron apoptosis; inflammatory response; vasculogenesis; neuromuscular junction development; aging; central nervous system neuron development; response to light stimulus; brain-derived neurotrophic factor receptor signaling pathway; adenylate cyclase activation; regulation of protein kinase B signaling cascade; learning; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system neuron development; positive regulation of phosphoinositide 3-kinase cascade; glutamate secretion; long-term memory; positive regulation of axonogenesis; cerebral cortex development; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Obesity, Hyperphagia, And Developmental Delay

48-Strip-Wells

435 USD

96-Strip-Wells

600