ELISA/assay

Human Paraoxonase ELISA Kit

MBS041476

48-Strip-Wells

435 USD

ELISA Kit

Human Paraoxonase ELISA Kit

Paraoxonase

paraoxonase; Serum paraoxonase/arylesterase 1; serum paraoxonase/arylesterase 1; K-45; PON 1; esterase A; A-esterase 1; paraoxonase B-type; aromatic esterase 1; arylesterase B-type; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1; paraoxonase 1; Aromatic esterase 1; A-esterase 1; K-45; Serum aryldialkylphosphatase 1

PON

PON1; PON1; ESA; PON; MVCD5; PON; PON 1

PON1_HUMAN

Human

This kit recognizes recombinant and natural Human PON, no significant cross-reactivity or interference was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Detection Range: 62.5 U/L - 2000 U/L. Sensitivity: 10 U/L.

Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Human PON in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Human PON concentration. The concentration of Human PON in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) are less than 15%. Inter-assay Precision: Inter-assay CV (%) are less than 15%.

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

4105841

AAD02576.1

P27169

39,731 Da

Phase I, Non P450 Pathway (198854)

The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008]

PON1: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. Belongs to the paraoxonase family. Protein type: Phosphatase (non-protein); Motility/polarity/chemotaxis; EC 3.1.1.81; Lipid-binding; Secreted; EC 3.1.1.2; Secreted, signal peptide; EC 3.1.8.1; Hydrolase. Chromosomal Location of Human Ortholog: 7q21.3. Cellular Component: extracellular space; intracellular membrane-bound organelle; extracellular region. Molecular Function: protein homodimerization activity; arylesterase activity; phospholipid binding; calcium ion binding; aryldialkylphosphatase activity. Biological Process: response to nutrient levels; response to external stimulus; dephosphorylation; organophosphate catabolic process; response to toxin; positive regulation of transporter activity; carboxylic acid catabolic process; positive regulation of binding; aromatic compound catabolic process; phosphatidylcholine metabolic process. Disease: Microvascular Complications Of Diabetes, Susceptibility To, 5

48-Strip-Wells

435 USD

96-Strip-Wells

600