ELISA/assay

Sheep Metallothionein 2 ELISA Kit

MBS041207

48-Strip-Wells

435 USD

ELISA Kit

Sheep Metallothionein 2 ELISA Kit

Metallothionein 2

Metallothionein 2; Metallothionein-2; metallothionein-2; MT-2; MT-II; metallothionein-2A; metallothionein-II; metallothionein 2A; Metallothionein-2A; Metallothionein-II

MT2

MT2A; MT2A; MT2; CES1; MT2; MT-2; MT-II

MT2_HUMAN

Sheep

No significant cross-reactivity or interference between Sheep MT2 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 mug/ml - 100 mug/ml. Sensitivity: 1.0 mug/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MT2 concentrations in Sheep serum, plasma and other body fluids. Using Purified Sheep MT2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MT2 and MT2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MT2 concentrations in Sheep serum, plasma and other body fluids. Using Purified Sheep MT2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MT2 and MT2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

1495466

CAA65915.1

P02795

6,042 Da

Cytokine Signaling In Immune System Pathway (366171); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Gamma Signaling Pathway (187106); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220)

MT2A: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. Belongs to the metallothionein superfamily. Type 1 family. Protein type: Carrier. Chromosomal Location of Human Ortholog: 16q13. Cellular Component: perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; zinc ion binding; drug binding. Biological Process: cellular copper ion homeostasis; cytokine and chemokine mediated signaling pathway; negative regulation of growth

48-Strip-Wells

435 USD

96-Strip-Wells

600