catalog number :
MBS041165
products type :
ELISA Kit
products full name :
Human Free Fatty Acid ELISA Kit
products short name :
Free Fatty Acid
other names :
free fatty acid receptor 2; Free fatty acid receptor 2; free fatty acid receptor 2; G protein-coupled receptor 43; G-protein coupled receptor 43; free fatty acid activated receptor 2; free fatty acid receptor 2; G-protein coupled receptor 43
other gene names :
FFAR2; FFAR2; FFA2R; GPR43; GPR43
uniprot entry name :
FFAR2_HUMAN
specificity :
No significant cross-reactivity or interference between Human FFA and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 50 umol/L ~ 1600 umol/L. Sensitivity: 10 umol/L.
other info2 :
Intended Uses: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate FFA concentrations in Human serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
products description :
Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate FFA concentrations in Human serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications!
ncbi acc num :
NP_005297.1
ncbi gb acc num :
NM_005306.2
ncbi mol weight :
37,144 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Free Fatty Acid Receptors Pathway (161023); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356); CAMP Signaling Pathway (1017634)
ncbi summary :
This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for short chain free fatty acids and may be involved in the inflammatory response and in regulating lipid plasma levels. [provided by RefSeq, Apr 2009]
uniprot summary :
FFAR2: Receptor for short chain fatty acids through a G(i)- protein-mediated inhibition of adenylyl cyclase and elevation of intracellular calcium. The rank order of potency for agonists of this receptor is acetate= propionate = butyrate pentanoate = formate. Belongs to the G-protein coupled receptor 1 family. Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: cell projection; integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; protein binding; lipid binding. Biological Process: fat cell differentiation; G-protein coupled receptor protein signaling pathway; sequestering of lipid; mucosal immune response; regulation of acute inflammatory response; cell surface pattern recognition receptor signaling pathway; positive regulation of cytokine production during immune response; positive regulation of acute inflammatory response to non-antigenic stimulus; glucose homeostasis; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production