ELISA/assay

Mouse Substance P Receptor ELISA Kit

MBS040699

48-Strip-Wells

435 USD

ELISA Kit

Mouse Substance P Receptor ELISA Kit

Substance P Receptor

substance-P receptor isoform short; Substance-P receptor; substance-P receptor; SPR; NK-1R; NK-1 receptor; substance P receptor; neurokinin-1 receptor; NK-1 receptor; NK-1R; Tachykinin receptor 1

SP-R

Tacr1; TACR1; TAC1R; TAC1R; SPR; NK-1R

NK1R_CAVPO

Mouse

No significant cross-reactivity or interference between Mouse SP-R and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SP-R concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse SP-R antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SP-R and SP-R antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SP-R in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SP-R concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse SP-R antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SP-R and SP-R antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SP-R in the samples is then determined by comparing the O.D. of the samples to the standard curve.

290491234

NP_001166333.1

NM_001172862.1

P30547

46,257 Da

Function: This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K. Subunit structure: Interacts with ARRB1 . By similarity. Subcellular location: Cell membrane; Multi-pass membrane protein. Sequence similarities: Belongs to the G-protein coupled receptor 1 family.

48-Strip-Wells

435 USD

96-Strip-Wells

600