Rat Estradiol, E2 ELISA Kit | MyBioSource MBS040105 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Estradiol, E2 ELISA Kit

catalog :

MBS040105

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS040105

products type :

ELISA Kit

products full name :

Rat Estradiol, E2 ELISA Kit

products short name :

Estradiol, E2

other names :

estradiol 17-beta-dehydrogenase 12; Estradiol 17-beta-dehydrogenase 12; estradiol 17-beta-dehydrogenase 12; 17-beta-HSD 12; 17beta-HSD type 12; 3-ketoacyl-CoA reductase; steroid dehydrogenase homolog; 17-beta-hydroxysteroid dehydrogenase 12; short chain dehydrogenase/reductase family 12C, member 1; hydroxysteroid (17-beta) dehydrogenase 12; 17-beta-hydroxysteroid dehydrogenase 12; 17-beta-HSD 12; 3-ketoacyl-CoA reductase

products gene name :

other gene names :

HSD17B12; HSD17B12; KAR; SDR12C1; 17-beta-HSD 12; KAR

uniprot entry name :

DHB12_HUMAN

reactivity :

Rat

sequence length :

312

specificity :

No significant cross-reactivity or interference between Rat E2 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 6.25 pg/ml - 200 pg/ml. Sensitivity: 1.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated E2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat E2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add E2 and E2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of E2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated E2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat E2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add E2 and E2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of E2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

7705855

ncbi acc num :

NP_057226.1

ncbi gb acc num :

NM_016142.2

uniprot acc num :

Q53GQ0

ncbi mol weight :

34,324 Da

ncbi pathways :

Androgen Biosynthesis Pathway (106154); Biosynthesis Of Unsaturated Fatty Acids Pathway (83034); Biosynthesis Of Unsaturated Fatty Acids Pathway (429); Fatty Acyl-CoA Biosynthesis Pathway (106115); Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway (413482); Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway (468402); Fatty Acid Elongation Pathway (82934); Fatty Acid Elongation Pathway (295); Fatty Acid Metabolism Pathway (868084); Fatty Acid Metabolism Pathway (878045)

ncbi summary :

This gene encodes a very important 17beta-hydroxysteroid dehydrogenase (17beta-HSD) that converts estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. [provided by RefSeq, Oct 2011]

uniprot summary :

HSD17B12: Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily. Protein type: Lipid Metabolism - androgen and estrogen; EC 1.1.1.62; Membrane protein, integral; Membrane protein, multi-pass; Lipid Metabolism - unsaturated fatty acid biosynthesis; Oxidoreductase. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: extracellular matrix; endoplasmic reticulum membrane; integral to membrane. Molecular Function: estradiol 17-beta-dehydrogenase activity; collagen binding; heparin binding; protein binding; fibronectin binding. Biological Process: extracellular matrix organization and biogenesis; triacylglycerol biosynthetic process; cellular lipid metabolic process; estrogen biosynthetic process; fatty acid biosynthetic process

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage