ELISA/assay

Canine Interleukin 34 ELISA Kit

MBS039863

48-Strip-Wells

435 USD

ELISA Kit

Canine Interleukin 34 ELISA Kit

Interleukin 34

interleukin-34 isoform 1; Interleukin-34; interleukin-34; interleukin 34

IL-34

IL34; IL34; IL-34; C16orf77; C16orf77; IL-34

IL34_HUMAN

Canine

No significant cross-reactivity or interference between Canine IL-34 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 pg/ml - 200 pg/ml. Sensitivity: 1.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-34 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine IL-34 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-34 and IL-34 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-34 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-34 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine IL-34 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-34 and IL-34 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-34 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

289666752

NP_001166243.1

NM_001172772.1

Q6ZMJ4

27,482 Da

Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008]

IL34: Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Belongs to the IL-34 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine; Secreted, signal peptide; Secreted; Cell cycle regulation. Chromosomal Location of Human Ortholog: 16q22.1. Cellular Component: extracellular space. Molecular Function: growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding. Biological Process: positive regulation of cell proliferation; innate immune response; positive regulation of protein amino acid phosphorylation; inflammatory response

48-Strip-Wells

435 USD

96-Strip-Wells

600