catalog number :
MBS039766
products type :
ELISA Kit
products full name :
Porcine Bone Morphogenetic Protein 2 ELISA Kit
products short name :
Bone Morphogenetic Protein 2
other names :
bone morphogenetic protein 2; Bone morphogenetic protein 2; bone morphogenetic protein 2; BMP-2; bone morphogenetic protein 2
products gene name :
BMP2
other gene names :
BMP2; BMP2; BMP-2; BMP-2
uniprot entry name :
BMP2_CHICK
specificity :
No significant cross-reactivity or interference between Porcine BMP2 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 6.25 pg/ml - 200 pg/ml. Sensitivity: 1.0 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated BMP2 concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine BMP2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add BMP2 and BMP2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BMP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated BMP2 concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine BMP2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add BMP2 and BMP2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BMP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_989689.1
ncbi gb acc num :
NM_204358.1
ncbi mol weight :
40,347 Da
ncbi pathways :
Hedgehog Signaling Pathway (83969); Hedgehog Signaling Pathway (474); Id Signaling Pathway (199059); TGF-beta Signaling Pathway (83970); TGF-beta Signaling Pathway (475)
uniprot summary :
Function: Negatively regulates the structure and function of the limb apical ectodermal ridge. Ref.2. Subunit structure: Homodimer; disulfide-linked . By similarity. Subcellular location: Secreted . By similarity. Sequence similarities: Belongs to the TGF-beta family.