Rat Apoptosis Inducing Factor ELISA Kit | MyBioSource MBS038920 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Apoptosis Inducing Factor ELISA Kit

catalog :

MBS038920

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS038920

products type :

ELISA Kit

products full name :

Rat Apoptosis Inducing Factor ELISA Kit

products short name :

[Apoptosis Inducing Factor]

other names :

[apoptosis-inducing factor; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor 1, mitochondrial; striatal apoptosis-inducing factor; programmed cell death 8 (apoptosis-inducing factor); apoptosis-inducing factor, mitochondrion-associated, 1; Programmed cell death protein 8]

products gene name :

[AIF]

other gene names :

[AIFM1; AIFM1; AIF; CMTX4; COWCK; PDCD8; COXPD6; AIF; PDCD8]

uniprot entry name :

AIFM1_HUMAN

reactivity :

Rat

specificity :

No significant cross-reactivity or interference between this analyte and analogues is observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Mouse Body Fluids And Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.625ng/ml-20ng/ml. Sensitivity: 0.1ng/ml.

other info2 :

Intra-assay Precision: Intra-assay CV (%) are less than 15%. Inter-assay Precision: Inter-assay CV (?) are less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determine the level of AGTRAP (hereafter termed "analyte") in undiluted original Mouse body fluids and tissue homogenates. This kit is NOT suitable for assaying non-biological sources of substances.

ncbi gi num :

55585894

ncbi acc num :

AAV54054.1

uniprot acc num :

O95831

ncbi mol weight :

66,901 Da

ncbi pathways :

Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Modulation By HSP70 Pathway (198877); Ceramide Signaling Pathway (138023)

ncbi summary :

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, May 2010]

uniprot summary :

PDCD8: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces parthanatos i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; EC 1.-.-.-; Oxidoreductase; Mitochondrial. Chromosomal Location of Human Ortholog: Xq26.1. Cellular Component: mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol; nucleus. Molecular Function: protein dimerization activity; protein binding; oxidoreductase activity, acting on NADH or NADPH; electron carrier activity; DNA binding; NAD(P)H oxidase activity. Biological Process: caspase activation; chromosome condensation; neuron differentiation; mitochondrial respiratory chain complex I assembly; neuron apoptosis; positive regulation of apoptosis; apoptosis; cell redox homeostasis; DNA fragmentation during apoptosis; DNA catabolic process. Disease: Cowchock Syndrome

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

680

size3 :

5x96-Strip-Wells

price3 :

3100

size4 :

10x96-Strip-Wells

price4 :

6095

more info or order :

MyBioSource product webpage