Fish Alanine Aminotransferase ELISA Kit | MyBioSource MBS038444 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Fish Alanine Aminotransferase ELISA Kit

catalog :

MBS038444

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS038444

products type :

ELISA Kit

products full name :

Fish Alanine Aminotransferase ELISA Kit

products short name :

Alanine Aminotransferase

other names :

alanine aminotransferase; Alanine aminotransferase 1; alanine aminotransferase 1; GPT 1; glutamic-alanine transaminase 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1; glutamic-pyruvate transaminase (alanine aminotransferase); Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1

products gene name :

ALT

other gene names :

GPT; GPT; AAT1; ALT1; GPT1; AAT1; GPT1; ALT1; GPT 1

uniprot entry name :

ALAT1_HUMAN

reactivity :

Fish

specificity :

No significant cross-reactivity or interference between Fish ALT and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 U/L - 100 U/L. Sensitivity: 1.0 U/L.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ALT concentrations in Fish serum, plasma and other body fluids. Using Purified Fish ALT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ALT and ALT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ALT in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ALT concentrations in Fish serum, plasma and other body fluids. Using Purified Fish ALT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ALT and ALT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ALT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

1507680

ncbi acc num :

BAA01186.1

uniprot acc num :

P24298

ncbi mol weight :

54,637 Da

ncbi pathways :

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (106173); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169)

ncbi summary :

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]

uniprot summary :

GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.2; Transferase. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: cytosol. Molecular Function: alanine transaminase activity; pyridoxal phosphate binding. Biological Process: amino acid biosynthetic process; L-alanine catabolic process

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage