ELISA/assay

Fish Metallothionein ELISA Kit

MBS038414

48-Strip-Wells

435 USD

ELISA Kit

Fish Metallothionein ELISA Kit

Metallothionein

metallothionein; Metallothionein; metallothionein; MT; metallothionein 2A; metallothionein 4

MT

MT4; MT; MT2A; MT

MT_CHICK

Fish

No significant cross-reactivity or interference between Fish MT and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 ?g/ml - 200 ?g/ml. Sensitivity: 1.0 ?g/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MT concentrations in Fish serum, plasma and other body fluids. Using Purified Fish MT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MT and MT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MT in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MT concentrations in Fish serum, plasma and other body fluids. Using Purified Fish MT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MT and MT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

46048711

NP_990606.1

NM_205275.1

P68497

6,462 Da

Function: Metallothioneins have a high content of cysteine residues that bind various heavy metals. Domain: Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines. Sequence similarities: Belongs to the metallothionein superfamily. Type 1 family.

48-Strip-Wells

435 USD

96-Strip-Wells

600