ELISA/assay

Rat Cyclin Dependent Kinase 9 ELISA Kit

MBS038244

48-Strip-Wells

435 USD

ELISA Kit

Rat Cyclin Dependent Kinase 9 ELISA Kit

Cyclin Dependent Kinase 9

cyclin-dependent kinase 9; Cyclin-dependent kinase 9; cyclin-dependent kinase 9; CDC2-related kinase; cell division protein kinase 9; serine/threonine protein kinase PITALRE; serine/threonine-protein kinase PITALRE; cell division cycle 2-like protein kinase 4; tat-associated kinase complex catalytic subunit; cyclin-dependent kinase 9; C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit

CDK9

CDK9; CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE; CDC2L4; TAK

CDK9_HUMAN

Rat

No significant cross-reactivity or interference between Rat CDK9 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 ng/ml - 200 ng/ml. Sensitivity: 1.0 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CDK9 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat CDK9 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CDK9 and CDK9 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CDK9 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CDK9 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat CDK9 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CDK9 and CDK9 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CDK9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4502747

NP_001252.1

NM_001261.3

P50750

42,778 Da

Disease Pathway (530764); Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway (161052); Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway (106004); Formation Of RNA Pol II Elongation Complex Pathway (105950); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984); HIV Transcription Elongation Pathway (106001); HIV Elongation Arrest And Recovery Pathway (161051)

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]

CDK9: a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol. Protein type: EC 2.7.11.23; Kinase, protein; Cell cycle regulation; EC 2.7.11.22; Nuclear receptor co-regulator; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK9 subfamily; CDK/CDK9 subfamily. Chromosomal Location of Human Ortholog: 9q34.1. Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; membrane; intracellular membrane-bound organelle; cytoplasm. Molecular Function: RNA polymerase subunit kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; chromatin binding; ATP binding; protein kinase activity. Biological Process: regulation of histone modification; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; viral reproduction; positive regulation of histone phosphorylation; positive regulation of viral transcription; regulation of mitotic cell cycle; DNA repair; protein amino acid phosphorylation; regulation of DNA repair; cell proliferation; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; replication fork arrest

48-Strip-Wells

435 USD

96-Strip-Wells

600