Human Hepatoma Derived Growth Factor ELISA Kit | MyBioSource MBS037283 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Hepatoma Derived Growth Factor ELISA Kit

catalog :

MBS037283

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS037283

products type :

ELISA Kit

products full name :

Human Hepatoma Derived Growth Factor ELISA Kit

products short name :

Hepatoma Derived Growth Factor

other names :

hepatoma-derived growth factor isoform a; Hepatoma-derived growth factor; hepatoma-derived growth factor; HMG-1L2; high mobility group protein 1-like 2; hepatoma-derived growth factor; High mobility group protein 1-like 2

products gene name :

HDGF

other gene names :

HDGF; HDGF; HMG1L2; HMG1L2; HDGF; HMG-1L2

uniprot entry name :

HDGF_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human HDGF and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HDGF concentrations in Human serum, plasma and other body fluids. Using Purified Human HDGF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HDGF and HDGF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HDGF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HDGF concentrations in Human serum, plasma and other body fluids. Using Purified Human HDGF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HDGF and HDGF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HDGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4758516

ncbi acc num :

NP_004485.1

ncbi gb acc num :

NM_004494.2

uniprot acc num :

P51858

ncbi mol weight :

26,788 Da

ncbi pathways :

Activation Of Chaperone Genes By XBP1(S) Pathway (530771); Activation Of Chaperones By IRE1alpha Pathway (105906); Metabolism Of Proteins Pathway (106230); Unfolded Protein Response Pathway (105904)

ncbi summary :

This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. This gene was thought initially to be located on chromosome X, however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

HDGF: Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. Belongs to the HDGF family. Protein type: Transcription regulation; Cytokine. Chromosomal Location of Human Ortholog: 1q23.1. Cellular Component: nucleoplasm; extracellular space; transcriptional repressor complex; cytoplasm. Molecular Function: heparin binding; growth factor activity; DNA binding; nucleotide binding. Biological Process: cell proliferation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; transcription, DNA-dependent; unfolded protein response; negative regulation of transcription from RNA polymerase II promoter; signal transduction

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage