catalog number :
MBS037143
products type :
ELISA Kit
products full name :
Human Platelet Factor 4 Variant 1 ELISA Kit
products short name :
Platelet Factor 4 Variant 1
other names :
platelet factor 4 variant; Platelet factor 4 variant; platelet factor 4 variant; PF4alt; PF4var1; C-X-C motif chemokine 4; platelet factor 4, variant 1 (PF4-like); platelet factor 4 variant 1; C-X-C motif chemokine 4 variant; CXCL4L1; PF4alt; PF4var1
products gene name :
PF4V1
other gene names :
PF4V1; PF4V1; PF4A; CXCL4L1; CXCL4V1; PF4-ALT; SCYB4V1; CXCL4V1; SCYB4V1
uniprot entry name :
PF4V_HUMAN
specificity :
No significant cross-reactivity or interference between Human PF4V1 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12ng/ml-100ng/ml. Sensitivity: 1.0ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PF4V1 concentrations in Human serum, plasma and other body fluids. Using Purified Human PF4V1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PF4V1 and PF4V1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PF4V1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PF4V1 concentrations in Human serum, plasma and other body fluids. Using Purified Human PF4V1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PF4V1 and PF4V1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PF4V1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_002611.1
ncbi gb acc num :
NM_002620.2
ncbi mol weight :
11,553 Da
ncbi pathways :
Cell Surface Interactions At The Vascular Wall Pathway (106062); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Common Pathway (106060); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028)
uniprot summary :
PF4V1: Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro). Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Chemokine; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q12-q21. Cellular Component: extracellular space. Molecular Function: heparin binding; chemokine activity; CXCR chemokine receptor binding. Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of leukocyte chemotaxis; immune response; response to lipopolysaccharide; inflammatory response; regulation of cell proliferation