catalog number :
MBS036835
products type :
ELISA Kit
products full name :
Fish beta Endorphin ELISA Kit
products short name :
beta Endorphin
other names :
Beta-endorphin
products gene name :
beta-EP
specificity :
No significant cross-reactivity or interference between Fish beta-EP and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 6.25 pg/ml - 200 pg/ml. Sensitivity: 1.0 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated beta-EP concentrations in Fish serum, plasma and other body fluids. Using Purified Fish beta-EP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add beta-EP and beta-EP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of beta-EP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated beta-EP concentrations in Fish serum, plasma and other body fluids. Using Purified Fish beta-EP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add beta-EP and beta-EP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of beta-EP in the samples is then determined by comparing the O.D. of the samples to the standard curve.