ELISA/assay

Monkey Soluble Interleukin 6 Receptor ELISA Kit

MBS036234

48-Strip-Wells

435 USD

ELISA Kit

Monkey Soluble Interleukin 6 Receptor ELISA Kit

Soluble Interleukin 6 Receptor

Soluble interleukin-6 receptor; Interleukin-6 receptor subunit alpha; interleukin-6 receptor subunit alpha; IL-6R 1; CD126 antigen; membrane glycoprotein 80; IL-6 receptor subunit alpha; interleukin 6 receptor; IL-6R 1; Membrane glycoprotein 80

SIL-6R

IL6R; IL6R; IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; gp80

IL6RA_HUMAN

Monkey

No significant cross-reactivity or interference between Monkey SIL-6R and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 15.6 pg/ml - 500 pg/ml. Sensitivity: 2.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SIL-6R concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey SIL-6R antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SIL-6R and SIL-6R antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SIL-6R in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SIL-6R concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey SIL-6R antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SIL-6R and SIL-6R antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SIL-6R in the samples is then determined by comparing the O.D. of the samples to the standard curve.

633863

AAC60635.1

P08887

51,548 Da

Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); HIF-1 Signaling Pathway (695200); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); IL-6 Signaling Pathway (198864); IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (106386); Interleukin-6 Signaling Pathway (205111)

This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.[provided by RefSeq, May 2011]

IL6R: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Belongs to the type I cytokine receptor family. Type 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, cytokine; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular space; basolateral plasma membrane; apical plasma membrane; plasma membrane; extracellular region; interleukin-6 receptor complex. Molecular Function: protein binding; enzyme binding; protein homodimerization activity; interleukin-6 binding; interleukin-6 receptor binding; ciliary neurotrophic factor receptor activity; interleukin-6 receptor activity. Biological Process: negative regulation of collagen biosynthetic process; positive regulation of smooth muscle cell proliferation; cytokine and chemokine mediated signaling pathway; neutrophil mediated immunity; positive regulation of interleukin-6 production; positive regulation of leukocyte chemotaxis; defense response to Gram-negative bacterium; endocrine pancreas development; positive regulation of chemokine production; activation of NF-kappaB transcription factor; positive regulation of tyrosine phosphorylation of Stat3 protein; monocyte chemotaxis; positive regulation of osteoblast differentiation; defense response to Gram-positive bacterium; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of MAPKKK cascade; response to cytokine stimulus; positive regulation of cell proliferation; negative regulation of interleukin-8 production; acute-phase response; hepatic immune response. Disease: Interleukin 6, Serum Level Of, Quantitative Trait Locus; Soluble Interleukin-6 Receptor, Serum Level Of, Quantitative Trait Locus

48-Strip-Wells

435 USD

96-Strip-Wells

600