ELISA/assay

Rat Oxidized Lowdensity Lipoprotein ELISA Kit

MBS035475

48-Strip-Wells

435 USD

ELISA Kit

Rat Oxidized Lowdensity Lipoprotein ELISA Kit

Oxidized Lowdensity Lipoprotein

oxidized low-density lipoprotein receptor 1 isoform 1; Oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1; hLOX-1; ox LDL receptor 1; lectin-type oxidized LDL receptor 1; scavenger receptor class E, member 1; C-type lectin domain family 8 member A; oxidized low-density lipoprotein receptor 1, soluble form; oxidized low density lipoprotein (lectin-like) receptor 1; C-type lectin domain family 8 member A; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1; Lectin-type oxidized LDL receptor 1

OXLDL

OLR1; OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1; CLEC8A; LOX1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1

OLR1_HUMAN

Rat

273

No significant cross-reactivity or interference between Rat OXLDL and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.5 ng/ml - 16 ng/ml. Sensitivity: 0.1 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated OXLDL concentrations in Rat serum, plasma and other body fluids. Using Purified Rat OXLDL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add OXLDL and OXLDL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of OXLDL in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated OXLDL concentrations in Rat serum, plasma and other body fluids. Using Purified Rat OXLDL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add OXLDL and OXLDL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of OXLDL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4505501

NP_002534.1

NM_002543.3

P78380

30,959 Da

Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450); Phagosome Pathway (153910); Phagosome Pathway (153859)

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

OLR1: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria. Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility. OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13.2-p12.3. Cellular Component: nucleoplasm; intracellular membrane-bound organelle; membrane; integral to plasma membrane; plasma membrane; receptor complex; lipid raft. Molecular Function: low-density lipoprotein receptor activity; protein binding; carbohydrate binding. Biological Process: cell death; receptor-mediated endocytosis; leukocyte adhesion; response to hydrogen peroxide; blood circulation; lipoprotein metabolic process; inflammatory response; proteolysis; blood coagulation; leukocyte migration. Disease: Myocardial Infarction, Susceptibility To

48-Strip-Wells

435 USD

96-Strip-Wells

600